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The remaining reads were merged using PEAR v0.9.659.
The paired-end sequences were overlapped and merged using FLASH58.
Illumina R1 and R2 reads are merged using FLASH18,19.
Biological replicates were then merged using samtools (Version 1.4).
These annotations were merged using the Cuffmerge algorithm to obtain the reference annotation for further analysis55.
Finally a group of outlier-rejected datasets was scaled and merged using XSCALE.
All data were indexed, integrated, scaled and merged using Mosflm and Scala52.
Paired-end reads were merged using FLASH36 and subsequently assembled in NEWBLER34.
Raw sequence reads were quality filtered using Trimmomatic and paired end reads were merged using the software PEAR.
Reads were merged using FLASH v.1.2.1137,39 and merged reads shorter than 450 bp were excluded.
Images were merged using Metamorph 6.1 imaging software (Downingtown, PA).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com