Figure 1b is Differential Interference Contrast (DIC) image and Fig. 1c is merged image of Fig. 1a and b.
Notably, 2 to 8% of cells in the colonies were identified as K14 and K19 double-positive (yellow in merge Figure 1B), a characteristic of putative breast stem/progenitors [ 56].
As expected, observation of the blue channel, corresponding to the MitoBlue emission, shows the typical mitochondrial network pattern; more importantly, the distribution of the stvAF594 conjugate is also consistent with mitochondrial targeting (red channel, Figure 4A, center) and fully coincident with that of MitoBlue (merge, Figure 4A, right).
Having Figure 2 after Figure 3 will also provide a better opportunity to integrate the two (currently neglected) panels in Figure 2 on mutant VCA's into the presentation of branching by mutant VCA's in Figure 4. B) Alternatively, consider merging Figure 2A,B (diVCAwt) with Figure 1 and merging the remaining panels in Figure 2B (diVCAmutants) with Figure 3.
The merge of Figures 2A and B (Figure 2C) is used to show the localisation of GdBN in cells.
Figure 4 Backward DIBR results and merged virtual image of "Book arrival" sequence.
Thus, all supplemental figures need to be fully merged with figures in the main body of the text.
Figures 4(Cix)– 4(Cxii) are control experiments showing DAPI staining and merged images of Figures 4(Ci)– 4(Civ) with 4(Cv)– 4C viii).
This is in agreement with results obtained with GdBN localised in Chinese Hamster Ovary (CHO) cells [23] and U87 cells [22]. Figure 4 Merge image of the transmission and fluorescence images obtained by confocal microscopy of U87 cell loaded with GdBN-Cy5.5 (red) at a concentration of 0.6 mM incubated for 12 hours.
By confocal microscopy, we have confirmed that Gremlin rapidly increased Smad 3 translocation to the nucleus; the latter demonstrated by the yellow nuclear staining observed in the merge of Figure 2, while in untreated cells, the nuclei are red.
