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We built a pipeline that merged contigs from each NGS data into hybrid assemblies.
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In the third and final stage of assembly we merge contigs from stage 2 along entire clone contigs using a specially designed assembler that uses the clone ordering and clone overlap information to optimize memory usage as well as reduce the number of potential overlaps examined.
When merging contigs from different clusters, some contig might be very similar or they can cover the same region of the genome, this can artificially increase these values.
Specifically, we reviewed those merged contigs resulted from Geneious post-assembly, one at a time, to corroborate that no mismatches were presented in these contigs.
It represents overlap information from these alignments in a graph, and then finds maximal paths in the graph to produced merged contigs.
Since a contig is represented as the MSA of its constituent reads (recall from section "General definitions, notations, and techniques"), the merged contig consists of all reads belonging to C l and C r.
HICF merged contig-end BAC pairs had average agarose band numbers that are not significantly different from the overall band number (18.02 vs. 18.15 in all BACs in contigs).
However they provide valuable information to merge contigs (reducing contig count from 146 to 122 and increasing N50 size from 99 Kbp to 150 Kbp) and scaffold them (resulting in 110 scaffolds with an N50 size of 232 Kbp), as well as provide indpendent verification of the assembly (266 reads spanned across Newbler contigs and 246 mates connected the contigs).
As we merge contigs we also take the union of the sets of clones.
After merging contigs in BCCs, all contigs were used for sequence variant analysis.
Merging contigs that overlapped with each other on the human genome resulted in 92,705 contig clusters.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com