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The merge image shows a complete overlap of the two signals.
A merge image clearly shows that the RNase A@C-dots can enter the cell via the endocytic route.
The merge image showed that a positive TUNEL reaction was observed only in vWF positive cells.
It is impossible to evaluate the extent of co-localization without the merge image and high resolution single-channel images.
Importantly, the merge image showed that a positive TUNEL reaction was observed only in vWF positive cells.
Especially in the color merge image (Fig. 4c) (Media 1) and the difference signal image (Fig. 4d) the biofilm and its spreading along the implant surface becomes visible.
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Single-channel and merge images were generated in Photoshop.
For the images presented in Fig. 2, a z-stack was acquired and an 'extended depth of field - EDF' algorithm58 was applied to merge images stacks into final display.
New video-processing chips, meanwhile, let agents merge images from infrared cameras with regular video.
Image J software was used to merge images.
DAPI staining (blue) was included in merge images.
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