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A dipodal rhodamine-based mercury complex have been designed and synthesized, for the selective detection of 3-mercaptopropionic acid (MPA).
In order to evaluate the effect of auxiliary binding groups on mercury complex formation, two dicysteinyl tripeptides, CXC, where C is cysteine and X is glycine (CGC) or glutamic acid (CEC) were reacted with mercury II) chloride.
The glutathione moiety is degraded in the bile duct and gall bladder to a dipeptide and finally to an L-cysteine mercury complex before entering the circulatory system [ 12].
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A study of the electrochemistry of mercury and organo mercury complexes with cysteine indicated the formation of stable complexes which can be utilized for the determination of the species in environmental matrices.
Second, inorganic mercury complexes with a number of ligands, particularly sulphydryl groups, causing inhibition of enzymes and protein transport mechanisms [ 2].
Regarding the ES-MS measurements (data not shown), the same MH+ ion cluster patterns are found for the four spiked mercury complexes as in the standards.
Certain species of mercury, namely small neutrally charged mercury complexes (e.g., HgS), enter sulfate-reducing bacteria through passive diffusion (Benoit et al. 1999a; Jay et al. 2002; Gilmour et al. 2011).
The high mobility of mercury in the body is attributed to the formation of water-soluble mercury complexes that are mainly, if not exclusively, attached to the sulfur atom of thiol groups such as glutathione [ 11].
The ratio between the molecular ions of the mercury complexes and those of the free reduced or oxidised thiols gives an indication for the reaction yield in solution, as well as the stability of the complexes.
Detection was done by online simultaneous coupling of ES-MS and inductively coupled plasma MS. When the mercury complexes were spiked in real samples (plant extracts), no perturbation of the separation and detection conditions was observed, suggesting that this method is capable of detecting mercury biothiol complexes in plants.
While no explanation can be given here for the intensity rise of the Hg2+ peak in the standard, in the shoot extract the formation of other, unidentified mercury complexes may be proposed.
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