Similar(60)
Approximately 40 million Solexa (Illumina Inc., San Diego, CA, USA) sequenced ESTs were also included from the 'digital transcriptome' of B. napus lines TapidorDH and Ningyou7 [24], which was assembled using Velvet [25] with minimum contig length of 100 bp, coverage cut-off of five and k-mer value of 23, producing 29,956 contigs.
Best assembly was found at K-mer value of 47 in case of Chiifu, Tetra and Candle and at K-mer value of 51 in case of YSPB-24.
The Illumina data were assembled using Velvet [ 55] at a k-mer value of 39 or 31, respectively.
The obtained transcripts were merged into a single set of sequences using the default K-mer value of 27.
Velvet is set with a k-mer value of 21 and a minimum contig length of 150 bp.
All of the assemblies used a k-mer value of 31, and the low quality first and last 5 bp of all reads were trimmed.
C. angaria Illumina reads of individual sequencing runs were assembled with Velvet 9 (ver. 1.2.03) using a k-mer value of 41.
Assembly at a K-mer value of 51 was found to be optimal for transcriptome assembly of both Heera and Varuna.
The authors also reported that Trinity run 20 times slower than Oases when the same k-mer value of 25 was used.
On the basis of these results, we used a k-mer value of 61 for de novo assembly of alfalfa EST reads.
Using 100 bp Illumina reads and a ~85 x genome coverage, a k-mer value of approximately 61 resulted in the highest N50 assembly.
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