Sentence examples for mer length of from inspiring English sources

In summary, a 25 mer length of homology, and a shorter insert size produced the highest yield of PCR products from the asymmetric bridge PCR reaction, and consequently, the highest yield of recombinant colonies.

CLC Genomics Workbench 6.0.1 (CLC Bio, Aarhus, Denmark) was used to discard poor-quality reads and/or terminal nucleotides at a threshold of Q13 (P = 0.05) after which de novo assembly was done using Velvet [ 31], and an optimal K mer length of 67 determined using VelvetOptimiser (http://bioinformatics.net.au/software.velvetoptimiser.shtml).shtml

The t7 phage metagenomic data was quality trimmed using a Phred [39], [40] Q20 score and the reads were assembled using Velvet 1.0.05 [41] with a K-mer length of 37, expected coverage of 25 and a coverage cutoff of 2, which produced assemblies containing the longest contigs.

Three de novo assembly software were used including SOAPdenovo with optimized k-mer length of 41, ABySS [ 31] with K-mer length of 49 and Trinity [ 32] (http://trinityrnaseq.sourceforge.net/) with default k-mer length of 25.

A k-mer length of 63 was selected for both SOAPdenovo2 and CLC Bio.

As a default, TagDust used a k-mer length of 12.

SOAPdenovo-Trans was similarly used, leaving a k-mer length of 31.

An optimized k-mer length of 25 was used to assemble the transcriptome.

In particular, the k-mer length of 25 could not be modified.

We used a k-mer length of 79 for contig assembly.

A k-mer length of 71 was chosen as the optimal value.