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In this example the k-mer length is specified as 7.
K-mer length is related inversely to the number of transcripts generated.
Empirically, the best sig-mer length is between 55 to 60, and we thus used 60 in other experiments.
Figure 2 shows how the k-mer length is closely related to the expression level of the transcripts being assembled.
The k-mer length is fixed to 35 because Oases is only capable of assembling these libraries on machines with 32 GB physical memory when k is large.
Our analysis confirms that restricting assemblies to only a single k-mer length limits the number of transcripts recovered, regardless of which k-mer length is chosen.
k-mer homoplasy is generally considered as "noise" in phylogenetic reconstruction [ 16] and is a particular problem when k-mer length is short and genome size is large.
Since the memory requirement of Oases exceeds 32 GB when the k-mer length is small, we fix k to 35.
One of the reasons for this result may be that k-mer length is fixed at 25 nt; such a small number may not be sufficient to discriminate among highly similar homeologous or even paralogous sequences.
As no optimal k-mer length is appropriate for all de novo transcriptome assemblies, the multiple k-mer method was used to obtain longer silver carp mRNA sequences, which are very useful in subsequent analysis steps.
So, a higher k-mer length will theoretically generate a more contiguous assembly of highly expressed RNAs while poorly expressed transcripts will be more easily obtained if a lower k-mer length is used.
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