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Using a K-mer distribution analysis, we estimated that quinoa has a genome size of apporoximately 1.45 Gbp (Supplementary information, Figure S1), which is consistent with previous estimates based on C-values14.
Distribution of all 64 trinucleotides (3-mers) was determined, and the 3-mer distribution in 1,000-bp 1,000-bpthat overlapped by half their length (500 bp) across the genome windowsputhat[39].
Thus, we can estimate the 5-mer distribution, instead of 4-mer distribution used in MetaCluster 4.0, to get a better binning result.
Thus, the 4-mer distribution, instead of 5-mer distribution used in the first round, is estimated based on each virtual contig for binning.
4-mer distribution analysis of gene mapped metagenomic sequences of serially diluted gut microbiota.
We furthermore demonstrate that k-mer distribution is associated with the quality of the metagenomic data.
Both tools use hash tables to compute the k-mer distribution for a given (fixed) k.
4-mer distribution analysis of barcode-cassette filtered metagenomic sequences of serially diluted gut microbiota.
The 5-mer distribution of each virtual contig is estimated and the virtual contigs are grouped using K-means clustering method based on the Spearman distance of the 5-mer distribution [Observation (C)].
Similar results were obtained using 4-mer or 6-mer distribution patterns (Additional file 1: Figure S14).
This way, longer contigs can provide the required k-mer distribution information, which will allow effective binning and taxonomic assignment.
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