Different MER designs were investigated.
AS and MER designed all experiments; FG and WBG designed two photon experiments.
MER designed the study, performed the statistical analysis and drafted the manuscript.
Chateau-sur-Mer, designed by a local architect, Seth Bradford, was built in 1852 and renovated by Richard Morris Hunt in 1872.
Two 3′,5′-bis-glycoconjugated 18-mers, designed for antisense experiments, have been prepared and their hybridization properties with a complementary DNA fragment evaluated by UV thermal analysis.
The Human Whole Genome One Array version 6 (Phalanx BiotechGroup, Taiwan) contains 32,679 DNA oligonucleotide probes, and each probe is a 60-mer designed in the sense direction.
The 12,288 unique array oligonucleotides were sense-strand 70-mers designed against open reading frames in the annotated CL Brener reference genome sequence and were printed in duplicate.
The 20 predicted sRNAs whose expression was detected by microarrays were reverse-transcribed using primers (18-mers) designed to match the predicted sRNA sequences immediately upstream of the transcription terminators.
The second set of capture probes was 26 oligos (1 49-mer and 25 50-mers) designed to target mcrA and mrtA (encoding the α-subunit of the methyl coenzyme M reductase isoform II), but not the mcrA of anaerobic methanotrophs (Supplementary Table S2, Supplementary Fig. S1).
