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Synthetic peptides (15 mer) corresponding to the HV3 region of SE-positive HLA-DR molecules likewise inhibit the pathway in HLA class-II negative cells.
The forward primer, G12F, was 19 mer, corresponding to nucleotide positions 169 187 (5′-GTT GTC GTC ATG CTG CCA T-3′), and the reverse primer, G12R, was 20 mer whose sequence was complementary to nucleotide positions 471 490 (5′-A GTA CAG TAC CAA ATT TCA T-3′).
To do this, we first transformed the number of oligomers calculated from the analysis explained above into protein mass using an average size for each oligomer class: 4-mer for 2 5 mers, 10-mer for 5 15 mers, and 30-mer for 15 150 mer (corresponding to the approximate size at which half of the total oligomers of that class is smaller and half bigger).
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The FASTA files of the nucleotide sequences for membrane and secreted proteins were processed twice using the program ArrayOligoSelector 3.8.1 http://.sourceforge.net/projects/arrayoligosel) to generate unique 50 mers corresponding to the 3' and 5' ends of the gene.
The two undecanucleotides (11-mers) corresponding to the XerC and XerD binding sites were designated in this study as dif XerC and dif XerD, respectively, whereas the central hexanucleotide between the two Xer binding sites was named as dif cent (Figure 1A).
It is important to guarantee that each k-mer corresponding to an insertion of a read alignment has a position in the reference genome.
The 5'-portion was a 25-mer corresponding to the adaptors A and B required by the 454 sequencing system and ending with the sequencing key "TCAG".
We show that injection of mice with DCs pulsed with a MUC1 tandem repeat peptide (60-mer corresponding to three tandem repeats) can protect wild-type and MUC1.Tg mice from subsequent challenge with a MUC1-expressing tumour.
We refer to the peak in the abundance spectrum that consists of the k-mers corresponding to single-copy sequence in the target genome as the principal peak.
We used a set of 53 oligonucleotides (35 mers) corresponding to highly represented clones (≥ 1%, see Methods) in hybridizations on high-density colony filters (See additional file 1).
If one considers a random sequence of t n-mers (corresponding to a "genome" length of N = nt base pairs), the number of n-mers that are absent in the sequence follows the classical coupon collector's paradigm [ 52].
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