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To each culture, 60 mL of 1 1 chloroform:methanol (CHCl3:MeOH) were added.
After 60-min incubation at room temperature, 200 μL of MeOH were added and vortexed for 10 s.
Tested oily solution and the same amount of MeOH were added in a separation funnel.
For a control, 15 mL of MeOH were added to 285 mL of water.
Assays were stopped by placing on ice after 300 μL MeOH were added.
After 30 min incubation at room temperature, 50 μl of phenylcyanide (100 ng μl-1 in MeOH) were added as internal standard and the mixture was extracted twice with 750 μl CH2Cl2.
Similar(53)
Mass spectra were collected in medium resolution and MeOH was added to enhance ionization.
Lithium acetate dihydrate (1.02 g, 10 mmol) in 10 mL of MeOH was added drop-wise to a solution of purpurin (2.56 g, 10 mmol) dissolved in 100 mL of methanol.
Every 10 minutes, approximately, the solvent dried and a new aliquot of MeOH was added.
After completion of the reaction, 10 mL of methanol (MeOH) was added in the reaction crude followed by filtration to remove the catalyst.
Following termination of fermentation, 10% methanol (MeOH) was added to the broth and homogenized to disrupt fungal mycelia followed by addition of an equal volume of dichloromethane (DCM).
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