Sentence examples for meoh for at from inspiring English sources

Planarians were treated with 2% HCl in PBS on ice for 3 5 min, fixed in Carnoy (60% Ethanol, 30% Chloroform, 10% Acetic acid) for 2 h at 4 °C on a shaker and stored in 100% MeOH for at least 2 h at −20 °C before being bleached in 5%H2O2/MeOHH for 16 20 h.

After the initiator was attached to the surface, the columns were flushed with MeOH for at least 20 column volumes.

After UV grafting, the BMA- co-EDMA- gr-AMPS columns were thoroughly flushed with MeOH for at least 40 column volumes to flush out any unreacted chemicals.

Dehydration was followed by two washes in 100% MeOH for at least 30 min each to ensure all residual PBS-T was removed.

For BrdU immunocytochemistry, the cells were then treated with MeOH for 10 min at 4 d, followed by 2 M HCl for 30 minutes at room temperature, and reacted with primary and secondary antibodies using standard protocols.

Then, the cells on the coverslips were fixed with 4% paraformaldehyde in PBS for 15 min, permeabilized with 80% MeOH for 6 min at −20°C, incubated with 0.1% ThS (Sigma, USA) for 5 min, and washed three times in ethanol (50%).

The air dried and powdered twigs (1 kg) were extracted with methanol (MeOH) for 48 h at room temperature.

The air dried and powdered stem bark (700 g) were extracted with methanol (MeOH) for 48 h at room temperature.

For IF analysis of WRAP53 overexpressing NIH 3T3 cells, cells were grown on sterilized cover slips and fixed with 100% MeOH for 20 min at −20°C.

The air dried and powdered sample (1 kg) from each plant was extracted with methanol (MeOH) for 48 h at room temperature.

Air-dried and powdered plant material was weighed (300 g) and soaked in 1 L of methanol (MeOH) for 48 h at room temperature.