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Individual steroid hormone stock solutions were prepared in methanol (MeOH) at a concentration of 1000 mg L-1 and kept at −20°C in amber vials for a maximum of three months.
Unless otherwise noted, all CD spectra were recorded in MeOH at a concentration of 0.5 mM, at 298 K, over a range of 300 200 nm, at a scan rate of 50 nm/min.
The extract was eluted in MeOH at a flow rate of 0.5 ml/min and 3 ml fractions were collected.
The mobile phase A was ultrapure water with 0.05 % FA and B was MeOH at a flow rate 0.4 mL/min.
This experiment yielded three fractions which were subjected to gel filtration in a 30 × 2 cm column filled with Sephadex LH-20 and eluted with MeOH at a flow of 0.5 ml/min.
Using Program 1 (H2O/ACN gradient), 44 fractions were collected in 30 s intervals, fractions were evaporated under a stream of nitrogen, and resuspended in MeOH at a concentration of 8 g seed equivalents/mL.
Similar(53)
The food additive samples were both extracted in 70% MeOH at an extraction ratio of 200 mg of biomass per 10 mL of 70% MeOH and centrifuged (4000 rpm, 15 min).
The hydro(solvo thermal reaction of Cd(NO3 2·4H2O and 2,6-naphthalenedicarboxylic acid (2,6-H2ndc) together with di 3-pyridylmethyl)amine, being as structure-di 3-pyridylmethyl N,N′-dimethylaminemide (DMAc)–MeOH at 120 °C afforded a cadmium–carbeingase network, which is formulated as [Cd3(2,6-ndc)3(DMAc)4] (1) by structure-direct-ray dinfraction analysis.
A single crystal of 1-MeOH mounted on an X-ray diffractometer was allowed to lose MeOH at room temperature under a dry nitrogen stream, during which time the unit cell of the crystal was monitored by X-ray diffraction and optical images of the crystal were recorded using a CCD microscope.
Subsequently, samples were dehydrated through a series of methanol (MeOH) diluted in PBS-T (25% MeOH, 50% MeOH, 75% MeOH, 100% MeOH) at RT while on a 3D rocker for 10 min (embryos) or 20 min (adult tissues) per solution.
Dried and finely powdered leaves and twigs (1.70 kg) of M. siamensis were percolated with MeOH at room temperature to produce a crude MeOH extract (215.00 g).
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