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The relative expression profiles of the above mentioned transcripts are depicted in Figure 6.
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All the above mentioned transcripts showed a minimum of 8 times enrichment in PGT and were confirmed by qRT-PCR.
Sequence of primers used to detect the telomeric and control transcripts are mentioned below.
As the current study is the first example of the use of monkey-derived ES cells in EST studies, the ES-specific transcripts are mentioned briefly (see Supplementary Table S3 where the contigs containing >5 ESTs are listed).
So, an absolute and a relative quantification of total HFE transcripts as well as of the two mentioned alternatively spliced transcripts were performed using samples of RNAs' pools from the diverse tissues.
A few "common" transcripts are worth mentioning.
Transcripts are heavily redacted.
Full transcripts are available here.
The transcripts are not released until May 2018.
As mentioned above, only 9 transcripts were common amongst tissues and spermatozoa, and the remaining ones restricted to a single tissue or sperm.
Some potentially ABA-inducible transcripts were already mentioned.
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CEO of Professional Science Editing for Scientists @ prosciediting.com