This observation could result from a different mode of regulation of these subunits, at least in the mentioned tissues.
Additionally, the liver from reindeer in the Finnish study represented the tissue with the clearest difference displaying the highest concentrations among the above mentioned tissues.
Formalin-fixed paraffin embedded (FFPE) tissues were obtained from the previously mentioned tissue banks in the form of 4 μm thick sections on slides.
The band of mouse VRK1 (51 kDa) was detected in thymus, spleen, and testis of wild type mice, but we could not detect any signal from the above-mentioned tissues in VRK1-deficient mice (Figure S2).
After cells were treated as mentioned in tissue culture for experimentation section, media from each of 24 wells containing secreted proteoglycans was collected and protease inhibitors (100 mM 6-amino caproic acid, 5 mM benzamidine hydrochloride) added.
As mentioned earlier, epidermal tissues in wheat straw are covered by a protective layer of cutin and waxes.
Also, we were not able to make in-depth comparisons of many of the tissues mentioned in our report as this was outside the scope of this study.
For example TLR4, which has also been mentioned in the tissue map analysis, is one of the most studied members of TLRs.
In addition to the gill cartilage chondrocytes mentioned earlier, other cartilaginous tissues in the radial cartilage of dorsal and anal fins, hyaline cartilage of the jaws and eyes were also Pis1-immunoreactive (see Figure 1a, c and Figure 5A-b).
The only place absorbance by nonblood tissues was mentioned was in question 3. Three students mentioned it in the prequiz, and a different three students mentioned it in the postquiz.
At E18.5, Creb1 protein was strongly detected in wildtype lung (as previously mentioned), however in lung tissue of E18.5 Creb1−/− mice no Creb1 immunoreactivity could be seen (Fig S1).
