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The likelihood of success of this approach for the plant acyl-ACP TEs is increased by the availability of the above mentioned sequence information as well as by a 3D structural model of the FatB enzyme [ 11].
As previously mentioned, sequence information from P. pachyrhizi is limited and mostly derived from cDNA libraries constructed from mRNA extracted from urediniospores and germinated urediniospores as well as from whole infected soybean leaves from 6 to 15 dai.
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Clearly, this kind of information can be applied to assemble the two P. edulis BACs that harbour the mentioned sequences.
In an analogous manner, the use of species-specific primers targeting other pathogens, such as those mentioned above for which extensive sequence information is available, would expand the diagnostic capability of the PCR-based molecular technology, i.e., the capability of speciation.
Among the above mentioned papers that mainly used the sequence information, some did not consider the sequence microenvironment [13] and some lacked a feature selection procedure [16].
Unfortunately, the ISH technique described here does not allow discrimination of different subgenera or species of haemosporidians, especially due to the previously mentioned lack of 18S rRNA gene sequence information of a large number of defined Plasmodium species.
However, none of the techniques mentioned above have been applied in litchi to date, simply because of the lack of genomic sequence information.
The sequence information is derived from the Illumina Adapter Sequences Document (1000000002694 v01000000002694
TARID sequence information is available from GenBank under KF484512.1.
Please see Supporting Table 1 for complete sequence information.
(Oligo sequence information is available upon request).
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