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The mentioned procedures are valid under the assumption that the paired laterals are laid on straight slopes; however, real microirrigation units rarely follow an even gradient, whose topography is characterized by equally spaced contour lines.
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All above mentioned procedures were carried out using SAS software version 8.02 [ 22].
Subsequently, any contigs that were not annotated in the above mentioned procedures were searched for the presence of open reading frames (ORFs) using the 'getorf' function in the EMBOSS package [ 38] with minimum nucleotide size of 100 between START and STOP codons.
Earlier mentioned procedure was performed at 0, 248 48, and 72 h.
The mentioned procedure is repeated, until certain stopping criterion is met (e.g., a fixed number of iterations).
The obtained solution of the above mentioned procedure is a Stackelberg equilibrium solution (Sakawa and Nishizaki 2009).
The above mentioned procedure is mainly based on the exchange of Initialization Messages (IMs) and allows to organize the network in clusters.
Tryptic digests of gel plugs that did not lead to identification of protein(s) using the above mentioned procedure were separated on a reverse phase ZORBAX 300SB-C18 (Agilent, Wilmington, DE USA) column on a multidimensional liquid chromatography system (Ettan MDLC, GE Healthcare, Uppsala, Sweden).
The above mentioned procedure was following the method of Alshawsh et al. [ 11].
The mentioned procedure was used only in case of comparisons of the spectra from hippocampal inclusions, normal tissue and creatine (see Figs. 1 and 4).
The above mentioned procedure was repeated, using 0.3 μg/ml primary antibody against ß-actin (cat. no. 8226, Abcam) for one hour at room temperature and 2 ng/ml goat anti-mouse secondary antibody conjugated with HRP (cat. no. 1858413, Pierce) for one hour.
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