Importantly, all the experiments were performed at 20°C and in the presence of cycloheximide (unless mentioned otherwise), to block new synthesis of GT-GFP and ST-RFP in the ER during the experiment and to block exit of these markers from the Golgi while enabling transport in the Golgi to proceed (Matlin and Simons, 1983).
All other chemicals unless mentioned otherwise were of analytical grade and used as received.
Unless mentioned otherwise, half of LC50, 8, 3.48, 0.33 and 5 mg/ml for PTZ, ETH, NaVP (all from Sigma-Aldrich), and LEV (Levesam 500, Nicholas Piramal), respectively, was used.
Unless mentioned otherwise, the numbering of amino acid residues refers to the sequence of apple (M. domestica) ACCO1.
For all our experiment described below unless mentioned otherwise, the number of Monte Carlo cycles is fixed to 100, the number of particles used is 500, and the size of the validation gate is 15.
Unless mentioned otherwise, the number of antenna doublets was set to M = 10.
Unless mentioned otherwise, two types of users are active in the area: voice call users (requiring 64 kbps) and data call users (requesting 1 Mbps) [31].
Unless mentioned otherwise 10 μg of RNA was loaded in each lane.
Unless mentioned otherwise, all animals assayed were of the hermaphrodite sex.
In our study, the hydrolytic activities of the LIPESV12 α/β-hydrolases were (if not mentioned otherwise) estimated colorimetrically in 500 μl of 50 mM potassium phosphate (K,P) buffer (pH 7.0), containing 0.1 % Triton X-100, substrate, and enzyme as described recently (Pérez et al. 2012; Tchigvintsev et al. 2014).
