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RNA was isolated with Nucleospin RNA isolation system (Macherey-Nagel, Duren, Germany) according to manufacturers' instructions, as mentioned in Material and Methods.
MRI signal abnormalities were scored as mentioned in Material and Methods (2.5).
It is based on clinical examination and radiological study as mentioned in material and method section.
Further studies with non-diagnostic aims but for identification of astrocytes co-locating with PrPsc were developed here, as mentioned in Material and Methods section.
As mentioned in material and methods, each herd could only contribute with one isolate per BPT and sampling occasion when comparisons between herds were made.
As mentioned in Material and methods, only one MRA had been excluded from the study because of major motion artefacts, but not because of insufficient arterial contrast or excessive venous enhancement.
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*** Predicted values as mentioned in Materials and Methods.
Histomorphometric analysis was performed, quantifying the parameters mentioned in "Materials and methods".
Therefore, we determined the adhesion ability of yeast isolates by following the protocol as mentioned in materials and methods.
The protein was expressed as inclusion bodies, which was later solubilised with urea as mentioned in materials and methods.
Mean values for total lipid and neutral lipid content (wt %) were determined as mentioned in Materials and Methods and did not differ significantly (p < 0.05).
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CEO of Professional Science Editing for Scientists @ prosciediting.com