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As already mentioned, alternatively to scrambling, we could use puncturing to achieve non-systematic coding and repeat the same comparative assessment, although we do not expect that different conclusions would result.
So, an absolute and a relative quantification of total HFE transcripts as well as of the two mentioned alternatively spliced transcripts were performed using samples of RNAs' pools from the diverse tissues.
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As previously mentioned, the alternatively spliced exon, lost in USP11, contains several reported phosphorylated serines in USP4 and USP15 [ 39].
However, the unresolved position of Semnopithecus among Asian colobines might have been affected by one or several of the above mentioned factors, or alternatively, might be the result of a true radiation-like divergence of lineages.
Alternatively, as mentioned, an arbitrator could take over all or part of the dispute from a mediator.
TPs with one or more AS events mentioned above were considered alternatively spliced and grouped as AS-TPs.
Alternatively, where mentioned, an equal volume of 4% PFA was carefully added to the medium without disturbing the cell culture within the incubator to avoid morphological changes due to the PBS wash.
Alternatively, as mentioned previously, our stringent filtering of variants was biased toward removing the SNPs most likely able to detect fine scale structure.
Alternatively, mentioning that you often stay at nearby hotel and are new to this one, gives the hotel incentive to impress you.
Alternatively, the already mentioned caged non-fluorescent organic dyes that can be activated upon irradiation with UV or IR light (Belov et al. 2010; Grimm et al. 2013) could be used in combination with a reducing and oxidizing system (ROXS), e.g., of 1 mM ascorbic acid and 1 mM methyl viologen under oxygen depletion (Vogelsang et al. 2008).
All ESTs showing these HSPs with the mentioned filtering criteria were named as ' alternatively spliced ESTs'.
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