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Only a few memory genes and a smaller number of down-regulated genes are shared between dehydration/ABA and other abiotic stress responding systems (Table 2; Additional file 5: Table S5; Figure 3).
Differentially expressed genes after conditioning were subsequently analysed by (1) a GO enrichment analysis, to assess functional expression patterns, (2) analyses of known (long-term) memory genes, and (3) identification of genes with an opposing differential expression pattern in N. vitripennis and N. giraulti.
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More than 200 JA-responsive genes are up-regulated in S1 (including memory and non-memory genes) and about 80 are down-regulated (Table 2; Additional file 5: Table S5).
The memory genes GLK1 and GLK2, implicated in regulating the photosynthetic apparatus and chloroplast development in a cell-autonomous manner [ 39, 40] are candidate genes for a role in such an adjustment.
For only two of the memory genes (dunce and S6KII), an antisense transcript in both N. vitripennis and N. giraulti was observed.
Mutations in the memory genes dunce and rutabaga increase visual responsiveness to moving gratings in an optomotor maze [3].
The learning and memory genes we tested and found to be involved are conserved across many animal species (Bolduc and Tully, 2009), and thus, serves as an excellent approach to model cellular and neuronal network functions that may be relevant to vertebrate brain function.
Sustained oviposition depression requires learning and memory genes in both teachers and students.
Concurrent with amplified responses from the and memory genes, however, engagement of the and genes lessens in S3, suggesting the nature and the dynamics of the interactions between dehydration and other signaling pathways are different during repeated exposures than the interactions occurring in a single exposure.
For example, a number of the memory genes encode chlorophyll and carbohydrate (starch) synthesizing enzymes, while chlorophyll and starch degrading activities are encoded by memory genes (Additional file 5: Table S5).
Species-specific distributions of the dehydration stress/JA co-regulated genes among the and memory genes (Table 2; Figure 6) and the memory genes encoding TFs in Z. mays and A. thaliana are displayed as well.
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