Probes and membrane were heated 85°C for 5 min, and probes were added to the membrane and incubated at 85°C for 10 min, followed by room temperature overnight.
Then the membrane was heated at 100 °C for 24 h.
The membrane is heated by an on chip platinum thin film heater.
The membrane was heated in a microwave at 50% power for 1 min.
The water is continuously added from an external source, wetting the hygroscopic hydrophobic membrane; the membrane is heated, yielding water from evaporation.
The seams between the pieces of membrane are heat- or solvent-welded together, and they are either ballasted with gravel or mechanically fastened to the underlying substrate, which is usually rigid foam insulation.
When protein ubiquitination was examined, membrane was heat-activated by autoclaving at 121°C for 35 min prior to blocking with nonfat dry milk solution to enhance antigenic site recognition.
All the membrane materials were heated for 3 h at 100 °C in a hot air oven for moisture removal.
For the stability assay, membranes containing 30 µg of protein were heated for 3 min at 95°C in Laemmli buffer (2% SDS, 10% glycerol, 100 mM DTT, 0.1% bromphenol blue, 62.5 mM Tris HCl, pH 6.8) containing 200 mM of the indicated chloride salt and immediately after were placed in ice.
