Dried membranes were exposed to a PhosphorImager screen, visualized by autoradiography and quantified using ImageQuant software.
Membranes were exposed digitally using the ChemiDoc MP System (Bio-Rad), and spots were quantified using the ImageJ software.
Membranes were exposed using the ECL method (GE Healthcare) according to the manufacturer's instructions.
The membranes were exposed to a number of media commonly utilized such as H2SO4, NaOH, K2Cr2O7, HNO3, etc.
Washed membranes were exposed to Phosphoimager screens.
Nitrocellulose membranes were exposed to autoradiography.
Membranes were exposed to a Phosphor-Imager screen at least overnight prior to vizualization.
After 1 minute incubation the membranes were exposed for 20 seconds to x-ray film.
The membranes were exposed overnight with intensifying screens and developed using a Molecular Imager FX (Biorad).
All the membranes were exposed on the same X-ray film.
After treatment with ECL reagent (Pierce), membranes were exposed to autoradiography film (Kodak).
