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Primers were labeled with biotin at the 5′-end; biotinylated MPCR products were detected by hybridization to the oligonucleotide probes immobilized on a membrane with subsequent colorimetric detection to confirm hybridization.
Increased intracellular Ca2+ levels in turn trigger the depolarization of the mitochondrial membrane with subsequent loss of membrane potential and elevated generation of ROS [95, 96].
PIP3 production causes Akt recruitment to the plasma membrane with subsequent Akt phosphorylation at Thr308 and Ser473 [3].
Angiogenesis is a complex process orchestrated by both growth factors and cell adhesion and is initiated by focal degradation of the vascular basement membrane with subsequent migration and proliferation of endothelial cells.
The proteins were separated by SDS-PAGE and transferred to a PVDF membrane with subsequent immunodectection using specific antibodies.
Increased intracellular Ca2+ levels in turn trigger the depolarization of the mitochondrial membrane with subsequent loss of membrane potential and elevated generation of ROS [ 95, 96].
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These radicals being electron deficient react with electron rich proteins to cause peroxidation of macromolecules like the membranes with subsequent leakage of the enzymes into the serum [21].
This can be due to the toxic effect of Amphotericin-B on the cartilage matrix as well as the synovial and capsular membranes with subsequent increase of synovial production [ 34].
The available data on stress-related degradation of chloroplast membranes with subsequent release of proteins to the cytoplasm suggests the hypothesis that they may be degraded outside chloroplasts under stress conditions [ 71].
Irreversible electroporation is a non-thermal ablation technique whereby electrical pulses delivered to tissue between two or more probes cause the creation of permanent cell membrane nanopores, with subsequent breakdown of cell membranes and death by apoptosis [ 5].
The fact that SMS is also leading to the induction of so-called touch genes leaves two possible scenarios open: i) modifications of the plant surface by SMS lead to a facilitated perception of DAMPs or MAMPs by membrane receptors with subsequent activation of defenses or ii) SMS is perceived by mechano-sensors that subsequently initiate resistance.
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wall with subsequent
mucosa with subsequent
film with subsequent
membrane with resultant
diaphragm with subsequent
membrane with long
membrane with variable
membrane with human
membrane with reconstituted
membrane with wt
membrane with two-step
membrane with tiny
membrane with high
membrane with double
membrane with macular
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