Sentence examples for membrane were purified from inspiring English sources

Periplasm and outer membrane were purified as described previously [44].

Laminin and matrigel, a reconstituted basement membrane, were purified from a Engelbreth Holm Swarm (EHS) tumour as described by Paulsson et al (1987) and Kleinman et al (1986), respectively.

Recombinant proteins corresponding to the N-terminal fragment, EssB-N, predicted to be localized in the cytoplasm and the C-terminal fragment, EssB-C, predicted to reside on the trans-side of the membrane, were purified with yields of 3 4 mg/l and 6 7 mg/l of bacterial culture respectively.

Next, the pellets (Fig. 3A, lanes 5 and 6) were resuspended and membranes were purified by floatation (Fig. 3B).

Hemichannel expressing Sf9 cell membranes were purified using our published protocol.

Plasma membranes were purified from microsomal fraction by partitioning in an aqueous polymer two-phase system [ 27].

Thylakoid membranes were purified from wild-type and npq4 leaves grown at different light and temperature conditions as previously described [ 33]. 1D and 2D SDS-PAGE was performed using a Tris-sulphate buffer system [ 34] in a 12 18% acrylamide gradient.

The solubilized membranes were purified by Ni affinity chromatography in 20 mM DPC and subsequently gel filtrated into a low DPC concentration (3.3 mM) in a Superdex200 column (GE).

Additionally, for gill tissue and gradient pellet samples, membrane proteins were purified from the enriched membrane fraction (see above) according to the protocol of Eymann et al. (2004) as described by Markert et al. (2007).

Plasma membrane proteins were purified from mouse LV or cell lysates using a MEM-PER Eukaryotic Membrane Protein Extraction kit (Thermo).

Membrane proteins were purified according to the method of Cornwell et al (1987).