Afterward, cells on the lower surface of the membrane were fixed with 4% paraformaldehyde and stained with crystal violet.
The migrated cells on the lower side of the membrane were fixed by 10% formalin and stained with eosin.
Adherent cells on polycarbonate membrane were fixed for 10 min using absolute ethanol and stained with Giemsa dye.
After treatment for 8 h, migrated cells on the lower membrane were fixed with 4% paraformaldehyde, stained with 0.5% crystal violet and counted by using Image J.
After incubation, cells that had entered the lower surface of the filter membrane were fixed with 4% paraformaldehyde for 25 min at room temperature.
Cells attached to the lower side of the membrane were fixed and stained.
The cells on the bottom part of the membrane were fixed in methanol containing 0.1% crystal violet.
Cells migrated through the membrane were fixed, stained with Giemsa stain (Sigma) and counted under light microscope.
Cells migrating thought the membrane pore and invading the underside surface of the membrane were fixed with 4% paraformaldehyde.
Cells that migrated through the pores sticking to the under surface of the membrane were fixed with 3.7% paraformaldehyde before staining with 0.2% crystal violet dye.
Cells having migrated through the pores to the lower surface of the membrane were fixed with methanol and stained with crystal violet.
