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Cells were incubated for 12 h, fixed in 75% ethanol for 10 min, and stained by crystal violet for 30 min. Cells that migrated cross the membrane were counted under a microscope from 6 randomly selected fields (at 200× magnification).
Cells migrated through polyester membrane were counted.
Cells migrating to the lower side of membrane were counted.
In this sense, only nuclei between the basal lamina and the plasmatic membrane were counted.
All retinal vascular cell nuclei anterior to the internal-limiting membrane were counted.
Migrated cells from the captured images per membrane were counted using NIH image software.
The invading cells on the entire membrane were counted under light microscope.
Cells adhering to the lower membrane were counted through an inverted microscope.
Five separate regions of the transwell membrane were randomly selected and the cells invading the membrane were counted manually.
Cells located at the basal membrane were counted under an Olympus BX51 photomicroscope equipped with an Insight QE Spot cameram.
The transfected cells in which over 70% of PHD-AKT fluorescence was located at the plasma membrane were counted as translocation -positive.
More suggestions(15)
layer were counted
barrier were counted
diaphragm were counted
mucosa were counted
membrane were analysed
membrane were visualized
membrane were placed
membrane were calculated
membrane were removed
membrane were tested
membrane were formed
membrane was counted
membrane were used
membrane were pressed
membrane were recorded
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