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Parts of the diffusion membrane were collected for scanning electron microscopy (SEM) analysis, and following post-intervention phase, the animals were euthanized under general anaesthesia using a single dose of pentobarbital (150 mg/kg).
To minimize positional effect of reporter gene expression, at least 1000 transformants on the membrane were collected to create a mixed population of transformants, which were cultured and assayed for the strength of GFP florescence.
Biopsies from N/R or from I areas of osteoarthritic synovial membrane were collected at the time of surgery.
The cells that had invaded through the collagen layer to attach to the polycarbonate membrane were collected and stained with a dye.
After a 24-hour incubation in a humidified incubator at 37°C with 5% CO2, cells that had invaded through the basement membrane were collected, stained, and counted by light microscopy [ 22].
Cells were incubated for 24 h at 37 °C and cells that invaded across the basement membrane were collected by incubating with extraction solution and stained per manufacturer's protocol.
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The process of making heparin begins with the intestines of slaughtered pigs, from which mucous membrane is collected and cooked, eventually producing a dry substance known as crude heparin.
Cell membrane was collected by ultracentrifugation at 150,000 g for 1 h.
The lysate was again centrifuged at 100 000 g for 20 min at 4°C, and the supernatant (membrane) was collected.
Plasma membrane was collected at the 0.85 M/1.2 M sucrose interface, and 30 μg of protein was loaded per lane for western blot analysis.
The cytoplasmic membrane was collected by centrifugation at 127 000 g for 2 h and then resuspended in a small volume of TAGS-buffer.
More suggestions(15)
bladder were collected
wall were collected
membrane were analysed
membrane were visualized
membrane were calculated
membrane were placed
membrane were removed
membrane were tested
membrane were formed
membrane were used
membrane were pressed
membrane were recorded
membrane were scraped
membrane were embedded
membrane was collected
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