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Lines and the artificial membrane were checked for clotting after dialysis.
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After microdialysis, the catheter was removed and the membrane was checked to ensure that no damaged had occurred.
The integrity of mitochondrial membrane was checked through the addition of 10 µM cytochrome c.
After removal, the microdialysis membrane was checked for potential blood clotting.
The membranes were checked using a 3×10−3 m2 flat cell, where the concentrated streams were recirculated to the feed reservoir.
The membranes were checked using a 3×10−3 m2 flat cell where the permeated and concentrated currents were recirculated to the feed reservoir.
The morphology and crystallinity of the as-prepared membranes were checked with scanning electron microscopy and X-ray diffraction.
Ponceau-S stained membranes were checked for equal protein loading.
We observed increased cleavage of PARP only at 24 h, membranes were checked for equal protein loading using β-actin as control.
The purity and integrity of membranes were checked by SDS gel electrophoresis on 10% acrylamide gels as described in ref (46) and immunoblotting with rabbit polyclonal anti-F1-α and anti-F1-β antibodies as described in ref (47).
Membranes were checked for successful protein transfer and loading control by incubation of the membranes for 1 min in ponceau S. The MS-MLPA is a semi-quantitative method for methylation profiling using a methylation-sensitive restriction enzyme (Nygren et al, 2005).
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