Sentence examples for membrane were blocked from inspiring English sources

The non-specific binding sites on the nitrocellulose membrane were blocked by incubation with 5% skim-milk powder in PBS/Tween for 1 h.

The residual binding sites on the membrane were blocked by incubating with 5% (w/v) nonfat dry milk in TBST (10 mM Tris, pH 8.0; 150 mM NaCl; 0.05% Tween 20) for 1 h at room temperature.

The non-specific sites on membrane were blocked with 1% BSA and then incubated with streptavidin conjugated to horseradish peroxidase (1∶2000) for 1 h at room temperature.

Following transfer, the membrane were blocked in TBST (TBS containing 0.1% Tween 20) containing 5% skimmed milk for 2 h, followed by incubation overnight at 4°C with appropriate primary antibodies.

Residual binding sites on the membrane were blocked in 5% skim milk for 1 h at room temperature.

Nonspecific antibody binding sites on the membrane were blocked with 5% skimmed milk in triethanolamine-buffered saline (TBS).

The membrane was blocked with blocking reagent for ELISA (Roche).

The membrane was blocked with Membrane Blocking Agent (GE Healthcare life sciences) and incubated with primary antibody at 4 °C overnight.

The membrane was blocked with 3% BSA in TBST buffer for 2 h.

The membrane was blocked by Tris-buffered saline Tween-20 (TBST) containing 5% milk for 1 h at room temperature.

For the determination of Hsp90 chaperone, the membrane was blocked with 5% milk in PBS containing 0.1%Tween-20Tween-20hr.