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The membrane was washed three times with PBST again.
Afterwards, the prepared VACNTs/PANi membrane was washed with water and then freeze-dried.
Subsequently, the membrane was washed with TBST buffer and incubated with horseradish peroxidase-conjugated secondary antibodies.
The PVDF membrane was washed 3 times for 10 min with PBS containing Tween-20 (PBST).
Then the membrane was washed and blocked in blocking solutions for 30 min at room temperature.
After each incubation, the membrane was washed in the same way.
Then, the membrane was washed three times and incubated in TMB membrane substrate for 5 min.
Membrane was washed as above.
The membrane was washed, and followed by enhanced chemiluminescence.
The membrane was washed three times with 0.1% TBST.
The membrane was washed three times with TTBS.
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