Sentence examples for membrane was saturated from inspiring English sources

This sharp interaction decreased by about 10-fold when the membrane was saturated with free peptide.

Beyond this concentration, the labeling in the plasma membrane was saturated, and the surplus probes were remained in the cytoplasm.

For blocking, the membrane was saturated with 3% bovine serum albumin (BSA) for 1 h at room temperature (RT), incubated with the primary monoclonal anti-zebrafish VEGF-A antibody (1∶200; R&D Systems, Wiesbaden, Germany) for 1 h and afterwards with horseradish peroxidase-conjugated anti-mouse IgG (1∶6250) for 45 min at room temperature.

The membrane was saturated with methanol and stained with 0.1% (w/v) Coomassie BBR250.

The membrane was saturated with 5%milk/PBS/0.1%Tween-20Tween-20

Membrane was saturated in blocking buffer phosphate buffered saline with 0.05% Tween-20 (PBS-T), 5% low-fat milk, 1% BSA and at 4°C overnight.

Unoccupied protein binding sites on the PVDF membrane were saturated by incubating with 5% dried milk (in TBS-T) for 1 h.

To prevent non-specific binding, the free binding sites of the membranes were saturated with 5%% fat free dry milk powder in TTBS (overnight at 4 °C with gentle agitation).

A likely explanation could be that the membranes are saturated with PDE3B proteins as a consequence of overexpression.

As expected for a specific interaction, the force values decreased to 75±25 pN when DRMs membranes were saturated with wtEBO16 (1 mM) (Fig. 4).

Membranes were saturated for 1 hr and incubated with primary Abs for 1 hr at room temperature or over night at 4°C.