Sentence examples for membrane was frozen from inspiring English sources

The membrane was frozen using liquid N2, and lyophilized.

Cells present on the nitrocellulose membrane were frozen in liquid nitrogen for 10 seconds and placed on Whatman paper filters pre-incubated with Z-buffer (8.52 g/L Na2HPO4, 5.5 g/L NaH2PO4, 0.75 g/L KCl and 0.12 g/L MgSO4) containing 0.18% (v/v) of 2-mercaptoethanol and 0.07% of bromo-4-chloro-3-indolyl B D galactosidase (X-gal).

In addition, samples of synovial membrane were frozen for later research.

The membranes were frozen before prior to SEM observation, followed by gold sputtering (Biorad Polaron Division Philips SEMEDAXAXL40L40; Pwith2/10) withethe potentials of 5 kV at different magnifications.

After a final centrifugation step at 48000 x g for 10 min the membranes were resuspended in 50 mmol/l Tris-HCl buffer, pH 7.4 and incubated with 2 U/ml adenosine deaminase (ADA) at 37°C for 30 min. The membranes were frozen in small aliquots and stored at −80°C until binding assay.

For unknown reasons, significant RNA degradation ensued when MSU-stimulated membranes, but not control membranes, were frozen and thawed before homogenization (data not shown).

The membrane preparation was frozen in liquid nitrogen and stored at −80 °C.

The pellet containing the lysosomal+plasma membrane fraction was frozen at −20 °CC for lipid and protein analysis.

The remaining tissue, mainly consisting of juice vesicles (endocarp), the segments with their membranes and the vascular bundles, was frozen under liquid nitrogen and stored at -80°C until used.

The nuclear suspension was stirred vigorously on ice for 30 min. The sample was centrifuged at 15 000 rpm for 12 min at 4°C, and the nuclear/membrane extract was frozen and stored at −80°C.

The saliva was frozen.