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After incubation, the membrane was fixed with Diff-Quick fixative and stained with Diff-Quick Solution I and II (DADE Behring Inc., Newark, DE).
The implantation procedure was performed through traditional arthroscopic portals, and the seeded membrane was fixed with fibrin glue, excluding water flow temporarily.
Each membrane was fixed on the circular opening of a permeation bottle (diameter = 1.5 cm, height = 5.0 cm) with the effective transfer area (A) of 1.33 cm2.
[16] A 25 mm × 70 mm polysulfone ultrafiltration membrane was fixed on the top of a microscope glass slide using a tape to prevent the membrane from rolling up and solution penetration through the edges.
After 24 hours, the medium was removed and the filter membrane was fixed with 4% formalin for 1 hour.
For whole membrane analysis, the membrane was fixed in 4% paraformaldehyde for 15 min inside the ALI transwell.
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Afterward, cells on the lower surface of the membrane were fixed with 4% paraformaldehyde and stained with crystal violet.
The migrated cells on the lower side of the membrane were fixed by 10% formalin and stained with eosin.
The elastic membrane is fixed around its boundary, with or without an initial pre-stretch, and may be initially curved prior to indentation.
Adherent cells on polycarbonate membrane were fixed for 10 min using absolute ethanol and stained with Giemsa dye.
After that, the edge of cellulose membrane is fixed firmly on the PET film by the scotch tape.
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