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The composite membrane was crosslinked by glutaraldehyde vapor to maintain its mechanical properties and fiber morphology in wet stage.
The membrane was crosslinked with a HL-2000 Hybrilinker at 1,200 J, and the blotted biotin-RNA was detected with Streptavidin-HRP (U-Cytech, CT353) and SuperSignal® West Pico Chemiluminescent Substrate.
Following transfer, this membrane was crosslinked and prehybridized at 35°C in Hyb buffer (50% formamide, 5×SSPE, 1% SDS, 5xDenhardt's solution, and 33 µg/ml denatured herring sperm DNA) for 1 2 hours.
The membrane was crosslinked and dried.
The membrane was crosslinked by ultraviolet radiation.
The membrane was crosslinked by UV light before hybridisation.
Similar(50)
This concept received strong recent support by experiments in which raft glycosphingolipid GM1 in lipid membranes was crosslinked using the pentavalent choleratoxin B subunit (CTB).
Then the composite SPPO membranes were crosslinked to decrease the methanol permeability and increase the mechanical properties, and the crosslinking time was controlled to determine the optimal crosslinking time.
Mixed matrix membranes of sodium alginate (NaAlg) and poly vinyl alcohol) (PVA) containing 5 and 10 wt.% silicalite-1 particles were fabricated by solution casting method and the cured membranes were crosslinked with glutaraldehyde.
Fourier transform infrared spectra (FTIR), proton nuclear magnetic resonance (1H NMR) and ultraviolet visible light spectra (UV vis) were used for structural characterization of modified chitosan, The obtained membranes were crosslinked by irradiation in the ultraviolet region, where the photosensitive monomers showed maximum sensitivity.
Membranes were crosslinked (240 mJ) using a UV Stratalinker 2400 (Stratagene).
More suggestions(15)
membrane was ruptured
membrane was determined
membrane was tugged
membrane was purchased
membrane was obtained
membrane was observed
membrane was coated
membrane was investigated
membrane was prepared
membrane was fabricated
membrane was stored
membrane was laid
membrane was applied
membrane was washed
membrane was designed
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