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A membrane fraction enriched in plasma membrane vesicles was prepared as described by Briskin et al. (1987) with minor changes.

Two Ax21-homologous proteins (Smlt0378 and Smlt0184) were found to be produced by S. maltophilia and their secretion via outer membrane vesicles was confirmed (Devos et al. 2015).

65Zn+2 uptake by brush border membrane vesicles was measured over 20 min with metal concentrations constant, and at 1 min with increasing metal concentrations.

The suspension containing membrane vesicles was layered over a 20%/30%/42% (w/w) discontinuous Suc gradient that contained, in addition to Suc, 10 mM Tris-HCl buffer (pH 7.6), 1 mM DTT, and 1 mM EDTA.

Association of vRNP with cellular membrane vesicles was also determined by membrane flotation assay.

The [3H]GABA level inside the plasma membrane vesicles was determined by liquid scintillation method.

Release of outer membrane vesicles is a common feature of Gram negative bacteria.

Brush-border membrane vesicles were prepared and sodium-dependent glucose uptake activity was measured using the rapid uptake/filtration technique.

Study Design: Syncytiotrophoblast microvillous membrane vesicles and basal membrane vesicles were isolated from uneventful pregnancies (control group, n = 32) and pregnancies complicated by gestational diabetes mellitus (n = 18).

Outer membrane vesicles were collected with an ISCO density gradient fractionator.

Membrane vesicles were collected by ultracentrifugation at 111,000×g for 2 hr at 4°C.