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Particularly, BIN1 is responsible for the membrane tubulation process in myocytes.
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Using time-lapse TIRFM we examined the dynamics of the processes of membrane tubulation and vesicle formation.
The role of BAR domains in membrane tubulation has opened-up the investigation of how membranes are remodeled during cellular processes.
As the sole cytokinetic F-BAR in S. cerevisiae, Hof1's inability to tubulate membranes is consistent with our conclusion that membrane tubulation by F-BAR proteins is not required during cytokinesis in yeast.
Three single point mutations in the BAR domain region have been shown to interfere with the membrane tubulation capacity of BIN1 and to lead to disorganized T-tubules.
Membrane tubulation, adhesion, vesiculation or bursting were observed with GUVs.
All processes mentioned have in common that they are dependent on the functional integrity and timely control of actin filament nucleation, elongation and associated motor protein activity in cell cortical regions that forms ruffles or membrane tubulation areas.
Unlike, FBP17 and CIP4, Toca-1 alone did not induce membrane tubulation.
This result suggests that N-WASP plays an important role in control of the membrane tubulation activity of Toca-1.
Coexpression of Toca-1W518K with N-WASP did not induce membrane tubulation or the formation of vesicles (Fig. 4B).
Data generated using Cyt. D also support a role for actin polymerization in membrane tubulation and vesicle formation - scission.
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