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In this case, β-cyclodextrin (4.0 mM) was supplemented as a cell membrane transfer agent; the culture was thoroughly shaken and then divided into 1.0 mL aliquots.
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The company's transfer agent is Computershare.
Electrophoresis, membrane transfer, hybridization, and washing as described before [28].
When gene copies are moved laterally, they are usually moved using a copy-and-paste mechanism (e.g. in plasmids, phage, class I transposons, outer membrane vesicles and gene transfer agents), not a 'stealing' mechanism such as cut-and-paste (e.g. class II Transposons, nanotubes [ 44]).
Possibly, these soil invertebrates are relevant "energy transfer agents".
19 Lentiviruses are highly efficient gene transfer agents.
Instead of the mixer/settler sequence of the conventional reactor, the aqueous and organic streams are contacted across a porous, hydrophobic membrane the role of the membrane is to stabilize the oil/water interface at a fixed position while affording efficient transfer of the phase-transfer agent.
After electrophoresis, proteins were transferred to PVDF membranes (Immobilon Transfer Membranes, Millipore, Schwalbach, Germany).
Proteins were electro-transferred onto nitrocellulose membranes in transfer buffer.
It can ably transfer neurotrophic agents to abolish neurodegenerative diseases.
SDS-PAGE gel was transferred onto a polyvinylidene difluoride (PVDF) membrane (Immobilon-P transfer membrane, Millipore) for immunoblot analysis.
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