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Evaluation of cyto- and genotoxicity in human lymphocytes was performed as well as hemolytic activity coupled with cellular membrane topography analysis in mammal erythrocytes.
A portion of the human type O blood was set aside for cell membrane topography analysis, while the other part was intended for isolation of erythrocytes.
Thus, two tests were performed, the classic hemolytic activity in erythrocyte suspension (2%) and the erythrocyte cell membrane topography analysis in whole human blood (type O).
The possibility of using cell membrane topography analysis via AFM was initially raised by Brand et al. [ 39], who showed marked changes in the erythrocyte membrane parameters caused by treatment with dermaseptins, which are cytotoxic peptides isolated from the skin of the frog Phyllomedusa hypochondrialis.
Among the cytotoxicity tests, evaluation of cytotoxicity and genotoxicity in human peripheral lymphocytes and hemolytic activity assay of mouse, rabbit, and human (types A, B, AB, and O) erythrocytes, coupled with cellular membrane topography analysis performed by atomic force microscopy (AFM), have been run.
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Therefore, the cellular membrane topography by AFM analysis can contribute with information about the integrity of the erythrocyte membrane exposed to Cry proteins.
Fujita, K. et al. Membrane topography of the hydrophobic anchor sequence of poliovirus 3A and 3AB proteins and the functional effect of 3A/3AB membrane association upon RNA replication.
Membrane topography resulted in random or aligned nanofibrous structures depending on the auxiliary collector setup used.
The concept of topography descriptor is a generalization originating from known techniques of three-dimensional surface topography analysis.
This paper proposes a new method for surface topography analysis based on empirical mode decomposition.
It gives guidance to the application of mechanical, microstructural, chemical characterization methods and topography analysis of materials.
More suggestions(15)
membrane recruitment analysis
membrane reactor analysis
membrane protein analysis
membrane receptor analysis
membrane integrity analysis
membrane proteome analysis
membrane image analysis
membrane filtration analysis
membrane oxygenation analysis
membrane viability analysis
membrane affinity analysis
membrane protrusion analysis
membrane disorder analysis
membrane array analysis
membrane expression analysis
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