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Self-assembly and fibrillization of Aβ42 on the membrane surface would be more in line with the observed binding, and we hypothesize that the specific Aβ-bound GM1 is acting as a seed for further Aβ molecules to aggregate.
In addition, increasing the feed flow rate or the smoothness of the membrane surface would be effective to avoid the membrane contamination by decreasing the risk of cells or macromolecules adsorbed on the surface of the membrane.
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At early time points, it is difficult to distinguish between the inclusion and bacterial membranes and a transient appearance at the inclusion surface would be difficult to detect.
It might be argued that the proposed docking surface of the VS is not likely to be available to a toxin in the membrane when the VS forms part of the intact ion channel, as a comparison with the x-ray structure of Kv1.2/2.1 (PDB code: 2R9R (57)) suggests that this surface would be largely hidden from the surrounding membrane by the pore domain.
Considering 22% particle sedimentation, at that particle concentration, the cellular surface would be more than completely covered by SNPs, indicating that membrane damage in absence of serum is initiated by direct contact of particles to the cellular surface accompanied by interaction of particles with membrane constituents.
This illustration shows a very slight difference in the heights of the crystal surface and a typical cell exposed on a porous membrane; in our set-up the QCM is 1.5 mm higher than an exposed cell surface would be.
The playing surface would be artificial turf.
Scanning of such a membrane with the colloid probe provides a direct visualisation of the membrane surface as would be experienced by a colloidal particle during filtration.
Although simple microscopic observation suggested that the mitochondrial inner membrane surface (MIMS) would be larger in differentiated cells, the increase was not found to be significant according to cardiolipin assay (Fig. 4F).
In the present study, levels of sCD40L were not directly associated with platelet count but were negatively associated with MCP-1, which could reflect a higher conservation of CD40L on the membrane surface, and therefore would be more liable to intercellular stimulation.
Secondly, a demonstration that the manufacture of membranes with a high surface potential would be very beneficial to the efficient operation of such processes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com