Sentence examples for membrane surface expression from inspiring English sources
Retrieval of the tagged proteins allowed us to compare total and membrane surface expression of Kv12.2 protein for each case.
Here we firstly demonstrate that KCNE1 and KCNE3 regulate the membrane surface expression of Kv12.2 in vitro.
In addition to determining the effect of KCNE peptides on Kv12.2 channel plasma membrane surface expression, we assessed changes in total channel protein expression.
Further studies are required to define whether KCNE1 and KCNE3 determine membrane surface expression of Kv12.2 through control of export to the plasma membrane, promotion of endocytic recycling, or a combination of both mechanisms.
In summary, this study demonstrates for the first time that KCNE1 and KCNE3 β-subunits form a tripartite complex with Kv12.2 channels in the brain; and furthermore suggests that regulation of membrane surface expression, and subsequent channel activation as a function of this association.
Here the P2Y2 receptor was found in the cell membrane (surface expression value 5.17±0.42; n = 3) whereas the ΔCTPxxP bestrophin-1 was found in the cytoplasm (surface expression value 1.29±0.03; n = 3) indicating an independent trafficking of the two proteins (p = 0.0008).
The IRFAP-HTS platform described in this study instead enables real-time HTS of membrane protein surface expression in an easy-to-use, versatile, and rapidly deployable format.
We identified SAV E2 as the temperature determinant by demonstrating that membrane trafficking and surface expression of E2 occurs only at low temperature and only in the presence of E1.
To test whether detaching adherent tumor cells with trypsin is suitable to measure the MICA/B membrane expression, the surface expression of MICA/B on cells that were detached with trypsin and accutase was compared by FACS analysis.
Together, these data indicate that pIN-G is a versatile, powerful, new reporter for analysing signals controlling membrane protein trafficking, surface expression and dynamics.
In these murine infection models, membrane CD69 interferes with surface expression of sphingosine 1-phosphate receptor 1 and blocks B-cell egress from particular lymphoid organs [34], [35].
