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This finding supports the idea that molecular recognition, not membrane surface charge, governs the architecture of the chloroplast thylakoid membrane.
Membrane surface charge is critical for the transient, yet specific recruitment of proteins with polybasic regions to certain organelles.
Biophysical analyses of peptide/bilayer membrane interactions point to distinct interactions of the analogues with lipid bilayers, and dependence upon membrane surface charge.
Not only do these chelating polymers change the membrane surface charge and pore size, but also provide an extra mean to remove heavy metal ions through adsorption in addition to traditional steric effect and Donnan exclusion.
Herein, a supported lipid bilayer platform was employed in order to investigate the effect of membrane surface charge (positive, negative, or neutral) on the adsorption of three complement proteins.
The membrane surface charge showed that the blended membrane has the highest charge at low and high pH with iso-electric point at pH 3 while there is no iso-electric point obtained for a controlled membrane.
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The in vitro study showed that β-lactams enhanced daptomycin bactericidal activity by means of a reduction in membrane surface charges.
The preferential interaction coefficients show on average −1.2 e membrane surface charges for the fast protein on ternary mixtures versus −1.0 e in the binary mixture accumulated near the macroion.
Additionally, it is possible to extend the therapeutic index of liposomal carriers over that of the corresponding conventional formulations by optimizing the lipid composition, liposomal size, membrane fluidity, surface charge, steric stabilization, and so forth.
With artificial membranes, lower surface charge result in less alamethicin inserted [ 70].
These results suggest that changes in membrane-negative surface charge due to protein or lipid modifications, which could occur during cell signalling, can regulate the binding of the Fyn-SH3 domain to MBP and thus could regulate the activity of Fyn at the OL plasma membrane.
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