Laminin staining remained indistinguishable from control tissue, indicating an intact basement membrane at this developmental time point.
In contrast, ARCα staining remained peripheral with or without urethane, as expected for a plasma membrane and/or vitelline layer (PMVL, Figure 1N) protein.
Lysosome staining remained relatively stable following LeTx stimulation until LysoTracker fluorescence declined gradually 6 9 min before the plasma membrane ruptured (Fig. 4a, b, Supplemental Fig. 5a and Supplemental Movie 9).
The low CD21-expressing (CD21lo) Namalwa and Daudi cells internalized anti-CD19 much better than the high CD21-expressors, but not as extensively as the CD21− cells, as judged by the remaining plasma membrane staining after 3 h Fig 1J,K) and 20 h (Fig 1R).
Three samples showed a moderate-to-strong membrane staining (i.e., 2+, 3+), whereas the remaining three showed weak staining (1+).
Membrane staining was accentuated in response to HAMLET (35 µM, 3 hours), but the stress fibers remained visible even after HAMLET treatment and there was no change in cell morphology or evidence of detachment.
In HER2-positive tumor samples containing low expression levels of CAV1, HER2 exhibits predominant membrane staining.
Strong cytoplasmic or membrane staining was considered positive.
Lymph node metastases from tumors positive for neu overexpression also showed the membrane staining pattern, whereas lymph node metastases from tumors negative for neu staining never did.
Membrane staining for EGFR.
