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2) Although the present study includes many biochemical data, the morphological evidence showing that IL-1β is indeed present in the inter-membrane space is very limited.
Supernatant (inter-membrane space) was collected, and pellet (mitoplasts) was resuspended in 100 μl of PP buffer.
Supernatant (mitochondrial matrix and inter-membrane space) was collected, and pellet (mitochondrial membranes) was resuspended in 100 μl of PP buffer.
As reported, all proteins of the mitochondrial inter-membrane space are synthesized in the cytosol and are then imported into the mitochondria [ 21].
The biological behavior of chondrocytes implanted within the MMH inter-membrane spaces was studied for 45 days in culture.
Since the FLAG-tagged proteins were protected from digestion to a comparable extent as peptidyl-prolyl cis-trans isomerase F (PPIF, a matrix protein), whereas proteins of the outer- and inter-membrane spaces were not, the MCU constructs are appropriately targeted with their carboxyl-termini facing the mitochondrial matrix.
It is localized to the inner membrane and intermembrane space, is constrained to the regions of the inner membrane that interact with the outer membrane, and is excluded from the cristae.
Multifold increase in the observed catalytic activity was registered for the catalytic membrane operated with the forced flow of the reaction solution through the membrane, as compared to the value achieved at the same conditions, but in the absence of the forced flow (i.e. when the membrane porous space was accessible to the reactants due to diffusion only).
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