Sentence examples for membrane solubilization techniques from inspiring English sources

Therefore, alternative membrane solubilization techniques are needed.

For instance, 2D-PAGE analysis in yeast failed to detect many integral membrane proteins (Santoni et al., 2000) requiring the use of specialized detergents and solubilization techniques.

Application of improvized protocols on the solubilization techniques with advance multidimensional LC separation should, in principle, allow us to scrutinize the E. coli membrane proteome with unparalleled sensitivity and accuracy.

Membrane solubilization and Ni2+-chelate affinity chromatography were carried out with each of these four detergents.

This fragment and a control protein located in the intermembrane space (IMS), OPA1, were digested upon membrane solubilization by increasing digitonin concentrations.

(iii) Cell membrane solubilization.

For plasma membrane solubilization Triton X-100 at final concentration of 0.25% was added.

After membrane solubilization in DM, MsbA purification was carried out using Ni-NTA chromatography.

Exposure for 30 min to skinning solution containing Triton X-100 1% was used to ensure membrane solubilization.

The extent to which membrane complexes can be investigated is limited by the membrane solubilization step, which will also interfere with protein protein interactions.

When the lipid bilayer is saturated, mixed micelles begin to form, resulting in the removal of phospholipids from the cell membranes and also membrane solubilization.