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The membrane sheets were subsequently stored in distilled water, containing 0.1%% formalin solution to prevent microbial growth.
Membrane sheets were fixed (and proteins immobilized) after membrane removal from cells [7], leading to equilibration of membrane and proteins prior to imaging.
Apical plasma membrane sheets were prepared, fixed with 4% PFA, 0.1% glutaraldehyde and labeled with affinity purified anti-mRFP antibody coupled directly to 5 nm gold as described previously [3], [4].
3T3-L1 cells were grown on glass coverslips, differentiated into adipocytes, and stimulated or not with insulin for 20 min. Plasma membrane sheets were prepared, immuno-stained with anti GLUT4 or anti TfR antibodies, and quantification of their amount was performed as previously described in [22], [43] using WGA labeling for normalization to the amount of membranes.
A minimum of 17 plasma membrane sheets were imaged and analyzed for each Ras mutant.
PVDF and PSF microfiltration membrane sheets were produced via the phase inversion procedure [ 22– 22].
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In practise, membrane modules, rather than flat membrane sheets, are used in industrial processes.
When the number of bipolar membrane sheets was 4, it was possible to control 4 the pH to under 4, a level at which lithium ions adsorbed onto LMO might affect the desorption phenomenon.
Methods for labeling proteins membrane sheets are described in Yang et al [ 41].
In the XT5 capsules 15 layers of flat membrane sheets are stacked with a bed height of 2.20 mm and a frontal area of 22.06 cm.
In axial flow configurations several membrane sheets are stacked in capsules whose diameter is several orders of magnitude larger than the bed length.
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