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Microbial presence in field water and different membrane samples was established by the following method.
The surface layer of the polyamide membrane samples was placed facing the crystal surface.
The pellet containing membrane samples was finally resuspended in the same buffer, frozen in liquid nitrogen, and stored at −80 °C until further use.
The abundance of MCT1 protein in post-nuclear membrane samples was determined by Western blotting as described previously (Dyer et al, 1997).
Spectral imaging of the different membrane samples was performed on a Zeiss LSM 780 confocal microscope equipped with a 32-channel GaAsP detector array.
The physical state of membrane samples was tested by using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), a non-polar compound inserting into the hydrophobic region of the bilayer [44].
Similar(54)
These dry membrane samples were used for SEM studies.
Before analysis, the membrane samples were prepared by degassing under vacuum at 30 °C for 2 h.
The PFA composite membrane samples were dried at 80°C overnight before the gas permeation test.
Before scanning, the membrane samples were coated with Au/Pd in a sputter-coater.
Membrane samples were characterized by several techniques to confirm the tailoring of membrane surfaces.
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