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In order to determine the exact metal content in the prepared nanocomposites, the membrane samples of known weight were treated with aqua regia to completely dissolve all MNPs.
The membrane samples of poly vinyl alcohol)/poly acrylic-acid) (PVA/poly acrylic-acidifferent draw ratios were studied by both 13C CPVA/PAAMR and wide-angle X-ray diffraction (WAXD) measurements.
PV B19 DNA was also detected in 22% of synovial membrane samples of children with JIA.
Expressions of β- D-hexosaminidase, β- D-glucuronidase, hyaluronidase, sperm adhesion molecule 1 and klotho genes were measured in synovial fibroblasts and synovial membrane samples of patients with rheumatoid arthritis and osteoarthritis by real-time PCR.
The detection of PKC α, p-PKC α, PKC ζ, and p-PKC ζ proteins was analyzed by densitometry and the amount of each target protein in each sample was normalized by the amount of β-actin present in each respective fraction as shown in Figures 8– 9. Consistently, Na+K+-ATPase was detected in only membrane samples of each experimental group.
DOI: http://dx.doi.org/10.7554/eLife.04135.003 To separate the precursor membranes active in LC3 lipidation as well as to determine the requirement of PI3K in generating them, we took membrane samples of untreated or starved Atg5 KO MEFs incubated with or without PI3K inhibitors.
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However, only little is known about the adsorption geometry of membrane samples on the SSM.
Similar to T-cells, membrane sample of erythrocytes lacked significant aHv1-N labeling in WB (not shown).
For plasma membrane samples, 100% of each peptide sample was labeled.
To separate inner and outer membranes, ∼320 μl of membrane samples were laid on top of 3 ml 18% Percoll (vol/vol in buffer A), followed by ultracentrifugation at 30,000 rpm in a TLA 100.3 rotor at 4°C for 15 min (Morein et al., 1994).
For experiments with membrane samples, equal amount of Triton X was added into all samples, normally the final concentration not exceeding 0.1% because of dilution.
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