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Animals were sacrificed at 3 months and analysis included diaphragm specific force measurements, membrane repair analysis in the FDB muscle and full necropsies to quantify dysferlin expression and assess histopathology.
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For histological analysis all muscles and organs (unless used for membrane repair) were embedded in 7% gum tragacanth and flash frozen in liquid nitrogen cooled isopentane.
An emergency response team for membrane repair.
Reddy, A., Caler, E. V. & Andrews, N. W. Plasma membrane repair is mediated by Ca2+-regulated exocytosis of lysosomes.
Changes in calcium levels within a cell regulate cellular responses as diverse as membrane repair and muscle contraction.
However, macrophages primed with IFNγ in the presence of C9433 had a significantly smaller fraction of PI-positive cells, suggesting that pretreatment with C9433 either reduced the membrane damage or enhanced the membrane repair.
S. cerevisiae endures inhibitors through different mechanisms, including detoxification by enzymatic reduction, efflux and membrane repair (Piotrowski et al. 2014).
The mechanism of lectin inhibition of membrane repair remains unclear.
Dysferlin was previously described to be involved in membrane repair.
For membrane repair to occur, the cell needs to patch the damaged membrane, and remodel the local cytoskeleton [26].
Dysferlin is a type II transmembrane protein implicated in surface membrane repair in muscle.
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